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== Lonza Group ==
Lonza was founded in Switzerland in 1897. It expanded to the United States in 1969. Lonza started its biotechnological business in 1980.<ref>{{REFweb
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Lonza offers Human Dermal Fibroblasts (NHDF). Clonetics™ Human Dermal Fibroblasts are available from either adult skin (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture.
Applications:
* ECM protein analysis
* Wound healing
* Collagen metabolism
* Cosmetics
* Skin therapy/models
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.<ref>{{REFweb
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| title=Human dermal fibroblasts
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Clonetics™ Dermal Fibroblast Cell Systems contain Normal Human Dermal Fibroblasts (NHDF) and optimized media for their growth. Each System can quickly generate NHDF cultures for the study of disorders of human fibroblasts such as fibrosis, scleroderma, fibrosarcoma, xeroderma pigmentosum and histiocytoma.<ref>{{REFweb
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| url=http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_ManualsProductInstructions_TechSheet_-_Dermal_Fibroblast_Cell_Systems_NHDF.pdf
| title=Lonza Dermal fibroblasts cell systems - techsheet
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→Cell Applications: typo
These companies offer foreskin cells online. This list is only a random sample taken from a search engine. More companies can be found searching for "human fibroblast neonate" or "human neonate keratinocytes".
== ATCC ==
ATCC maintains nearly 4,000 cell lines for public health research, including lines used for the study of cancer, such as HeLa cells, as well as over 70,000 microbial cultures. The items on their repository have been used for the discovery, invention, and development of new, improved and emerging advances in the biological sciences arena.<ref>{{REFweb
| quote= | url=http://www.atcc.org/en/About/About_ATCC/Ethical_Standards_for_Obtaining_Human_Materials.aspx | title=ATCC Ethical standards for obtaining human materials | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
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ATCC offers BJ (ATCC® CRL-2522™), fibroblasts from human neonate foreskin. The cells may be used for stable transfection studies.<ref>{{REFweb
| quote= | url=http://www.atcc.org/Products/All/CRL-2522.aspx | title=BJ (ATCC CRL-2522) | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
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== Cell Applications ==
From an advertisement video:
{{Citation
|Text=At Cell Applications, Inc., primary Human Dermal Fibroblasts are isolated from the dermis of normal human neonatal [[foreskin]] or adult [[skin]]. They are cryopreserved at the end of primary culture and can be cultured and propagated for at least 16 population doublings. The cells are tested positive for fibroblast surface protein, and can be shipped frozen or proliferating, with all the media reagents included. HDF provide a well-established system for in vitro analysis of fibroblast growth, migration and collagen metabolism. Fibroblasts grown in a biodegradable mesh have been used as a living dermal replacement.
|Author=Cell Applications, Inc.
}}
<br>
: <youtube>V4tmi_Ah1uI</youtube>
== Life Technologies ==
Life Technologies, a Thermo Fisher Scientific Brand, offers Human Dermal Fibroblasts, neonatal (HDFn) part number C-004-5C, described as primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture, for research use only. Cryopreserved HDFn are intended for use by researchers investigating the molecular and biochemical bases of
various normal and disease processes. Not for use in diagnostic procedures.<ref>{{REFweb
| quote= | url=https://www.lifetechnologies.com/order/catalog/product/C0045C | title=Catalog, product C0045C | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
}}</ref> <ref>{{REFweb
| quote= | url=https://tools.lifetechnologies.com/content/sfs/manuals/HDFn_man.pdf | title=Life Technologies HDFn manual | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
}}</ref>
Life Technologies, now a brand of Thermo Fisher Scientific, traces its roots back to 1962 with the creation of Grand Island Biological Company (GIBCO) in Grand Island, New York, making serum harvested from horses raised on their land. The original Life Technologies, Inc., a subsidiary of Dexter Corp., was founded in 1983 by the merger of Bethesda Research Laboratories, Inc. and GIBCO Corporation. In 2000, Life Technologies was bought out by Invitrogen. Eight years later, following Invitrogen Corporation’s acquisition of Applied Biosystems Inc., Invitrogen renamed itself, creating the new Life Technologies Corp. In 2014, Life Technologies became the fourth premier brand within Thermo Fisher, the world leader in serving science.<ref>{{REFweb
| quote= | url=https://www.lifetechnologies.com/us/en/home/about-us/news-gallery/company-fact-sheet/company-history.html | title=Life Technologies, company history | last= | first= | publisher= | workdate= |accessdate=2015-06-30}}</ref> == Lonza Group == Lonza was founded in Switzerland in 1897. It expanded to the United States in 1969. Lonza started its biotechnological business in 1980.<ref>{{REFweb |quote= |url=http://www.lonza.com/about-lonza/company-profile.aspx |title=Lonza, company profile |last= |first= |publisher= |date= |accessdate=2015-06-30}}</ref> Lonza offers Human Dermal Fibroblasts (NHDF). Clonetics™ Human Dermal Fibroblasts are available from either adult [[skin]] (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult [[skin]]. They are cryopreserved at the end of primary culture. Applications:* ECM protein analysis* Wound healing* Collagen metabolism* Cosmetics* Skin therapy/models All cells test negative for mycoplasma, bacteria, yeast, and fungi. [[HIV]]-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.<ref>{{REFweb |quote= |url=http://www.lonza.com/products-services/bio-research/primary-cells/human-cells-and-media/fibroblasts-and-media/human-dermal-fibroblasts.aspx |title=Human dermal fibroblasts |last= |first= |publisher= |date= |accessdate=2015-06-30}}</ref> Clonetics™ Dermal Fibroblast Cell Systems contain Normal Human Dermal Fibroblasts (NHDF) and optimized media for their growth. Each System can quickly generate NHDF cultures for the study of disorders of human fibroblasts such as fibrosis, scleroderma, fibrosarcoma, xeroderma pigmentosum and histiocytoma.<ref>{{REFweb |quote= |url=http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_ManualsProductInstructions_TechSheet_-_Dermal_Fibroblast_Cell_Systems_NHDF.pdf |title=Lonza Dermal fibroblasts cell systems - techsheet |last= |first= |publisher= | date= | accessdate=2015-06-30
}}</ref>
== PromoCell ==
PromoCell offers Primary Normal Human Dermal Fibroblasts (NHDF), isolated form the dermis of juvenile foreskin or adult [[skin ]] from different locations like the face, the breasts, the abdomen, and the thighs. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.<ref>{{REFweb | quote= | url=http://www.promocell.com/products/human-primary-cells/fibroblasts/normal-human-dermal-fibroblasts-nhdf/ | title=Promocell - Normal human dermal fibroblasts NHDF | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
}}</ref>
According to PromoCell's website, the tissue used by PromoCell for the isolation of human cell cultures is derived from donors who have signed an [[informed consent ]] form (this being done by the donor himself, an authorized agent, or a legal agent) which outlines in detail the purpose of the donation and the procedure for processing the tissue. We do not accept or use any tissue without prior signing of the consent documents.
When obtaining and using human tissue, PromoCell acts in strict compliance with
* "German Federal Data Protection Act“ to protect privacy of donors.<ref>{{REFweb
| quote= | url=http://www.promocell.com/products/ethical-standards/ | title=PromoCell ethical standards | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
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ZenBio page on Human Dermal Fibroblasts states that the "cells are isolated from adult abdominal dermal explants". However, the subsequent list of products includes item DFN-F, "Cryopreserved, Neonatal Dermal Fibroblasts (0.5 x 106 cells). Includes 50ml of DF-1 growth medium"<ref>{{REFweb
| quote= | url=http://www.zen-bio.com/products/cells/fibroblasts.php | title=ZenBio Fibroblasts | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
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The Human Adult Dermal Fibroblasts Manual, on the very first page, states: "Human Adult or Neonatal Dermal Fibroblast Cells", and explains in the introduction: "Neonatal dermal fibroblasts are isolated from the foreskins of healthy male newborns. The cells are isolated by centrifugal force following enzymatic treatment or from an explant culture."<ref>{{REFweb | quote= | url=http://www.zen-bio.com/pdf/ZBM%20Human%20Adult%20Dermal%20Fibroblast%20Manual.pdf | title=Human Adult Dermal Fibroblasts Manual | last= | first= | publisher= | work= | date= | accessdate=2015-06-30
}}</ref>
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[[Category:Foreskin Productsproduct]] [[Category:Accepted plural name category]]
[[Category:From IntactWiki]]