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== Lonza Group ATCC == ATCC is a private, nonprofit biological resource center (BRC) and research organization whose mission focuses on the acquisition, authentication, production, preservation, development and distribution of standard reference microorganisms, cell lines and other materials for research in the life sciences. ATCC was established in 1925 when a committee of scientists recognized a need for a central collection of microorganisms that would serve scientists all over the world. ATCC activities are housed in a Manassas, Virginia facility, since 1998.

Lonza was founded in Switzerland in 1897ATCC maintains nearly 4,000 cell lines for public health research, including lines used for the study of cancer, such as HeLa cells, as well as over 70,000 microbial cultures. It expanded to The items on their repository have been used for the United States discovery, invention, and development of new, improved and emerging advances in 1969. Lonza started its biotechnological business in 1980the biological sciences arena.<ref>{{REFweb

|url=http://www.lonzaatcc.comorg/about-lonzaen/company-profileAbout/About_ATCC/Ethical_Standards_for_Obtaining_Human_Materials.aspx |title=Lonza, company profileATCC Ethical standards for obtaining human materials

Lonza ATCC offers Human Dermal Fibroblasts BJ (NHDF). Clonetics™ Human Dermal Fibroblasts are available from either adult [[skin]] (NHDFATCC® CRL-Ad2522™) or neonatal foreskin (NHDF-Neo). They are derived , fibroblasts from the dermis of normal human neonatal neonate foreskin or adult [[skin]]. They are cryopreserved at the end of primary culture. Applications:* ECM protein analysis* Wound healing* Collagen metabolism* Cosmetics* Skin therapy/models All The cells test negative for mycoplasma, bacteria, yeast, and fungi. [[HIV]]-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided may be used for each cell lot purchasedstable transfection studies.<ref>{{REFweb

|url=http://www.lonzaatcc.comorg/products-services/bio-researchProducts/primary-cellsAll/humanCRL-cells-and-media/fibroblasts-and-media/human-dermal-fibroblasts2522.aspx |title=Human dermal fibroblastsBJ (ATCC CRL-2522)

Clonetics™ Dermal Fibroblast == Cell Applications == From an advertisement video:{{Citation |Text=At Cell Systems contain Normal Applications, Inc., primary Human Dermal Fibroblasts (NHDF) are isolated from the dermis of normal human neonatal [[foreskin]] or adult [[skin]]. They are cryopreserved at the end of primary culture and can be cultured and propagated for at least 16 population doublings. The cells are tested positive for fibroblast surface protein, and optimized can be shipped frozen or proliferating, with all the media reagents included. HDF provide a well-established system for their in vitro analysis of fibroblast growth, migration and collagen metabolism. Fibroblasts grown in a biodegradable mesh have been used as a living dermal replacement. Each System can quickly generate NHDF cultures for the study of disorders of |Author=Cell Applications, Inc.}} <br>: <youtube>V4tmi_Ah1uI</youtube> == Life Technologies == Life Technologies, a Thermo Fisher Scientific Brand, offers Human Dermal Fibroblasts, neonatal (HDFn) part number C-004-5C, described as primary human dermal fibroblasts such as fibrosisisolated from neonatal foreskin, sclerodermacryopreserved at the end of the primary culture, fibrosarcoma, xeroderma pigmentosum for research use only. Cryopreserved HDFn are intended for use by researchers investigating the molecular and biochemical bases of various normal and histiocytomadisease processes. Not for use in diagnostic procedures.<ref>{{REFweb

|url=httphttps://biowww.lonzalifetechnologies.com/uploadsorder/tx_mwaxmarketingmaterialcatalog/Lonza_ManualsProductInstructions_TechSheet_-_Dermal_Fibroblast_Cell_Systems_NHDF.pdfproduct/C0045C |title=Lonza Dermal fibroblasts cell systems - techsheetCatalog, product C0045C

}}</ref> == ATCC == ATCC is a private, nonprofit biological resource center (BRC) and research organization whose mission focuses on the acquisition, authentication, production, preservation, development and distribution of standard reference microorganisms, cell lines and other materials for research in the life sciences. ATCC was established in 1925 when a committee of scientists recognized a need for a central collection of microorganisms that would serve scientists all over the world. ATCC activities are housed in a Manassas, Virginia facility, since 1998.  ATCC maintains nearly 4,000 cell lines for public health research, including lines used for the study of cancer, such as HeLa cells, as well as over 70,000 microbial cultures. The items on their repository have been used for the discovery, invention, and development of new, improved and emerging advances in the biological sciences arena.<ref>{{REFweb

|url=httphttps://wwwtools.atcclifetechnologies.orgcom/encontent/Aboutsfs/About_ATCCmanuals/Ethical_Standards_for_Obtaining_Human_MaterialsHDFn_man.aspxpdf |title=ATCC Ethical standards for obtaining human materialsLife Technologies HDFn manual

ATCC offers BJ Life Technologies, now a brand of Thermo Fisher Scientific, traces its roots back to 1962 with the creation of Grand Island Biological Company (ATCC® CRL-2522™GIBCO)in Grand Island, fibroblasts New York, making serum harvested from human neonate foreskinhorses raised on their land. The cells may be used for stable transfection studiesoriginal Life Technologies, Inc., a subsidiary of Dexter Corp., was founded in 1983 by the merger of Bethesda Research Laboratories, Inc. and GIBCO Corporation. In 2000, Life Technologies was bought out by Invitrogen. Eight years later, following Invitrogen Corporation’s acquisition of Applied Biosystems Inc., Invitrogen renamed itself, creating the new Life Technologies Corp. In 2014, Life Technologies became the fourth premier brand within Thermo Fisher, the world leader in serving science.<ref>{{REFweb

|url=httphttps://www.atcclifetechnologies.orgcom/Productsus/Allen/CRLhome/about-2522us/news-gallery/company-fact-sheet/company-history.aspxhtml |title=BJ (ATCC CRL-2522)Life Technologies, company history

== Life Technologies Lonza Group ==

Life Technologies, a Thermo Fisher Scientific Brand, offers Human Dermal Fibroblasts, neonatal (HDFn) part number C-004-5C, described as primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture, for research use onlyLonza was founded in Switzerland in 1897. Cryopreserved HDFn are intended for use by researchers investigating It expanded to the molecular and biochemical bases of various normal and disease processesUnited States in 1969. Not for use Lonza started its biotechnological business in diagnostic procedures1980.<ref>{{REFweb

|url=httpshttp://www.lifetechnologieslonza.com/orderabout-lonza/catalog/product/C0045Ccompany-profile.aspx |title=CatalogLonza, product C0045Ccompany profile

}}</ref>  Lonza offers Human Dermal Fibroblasts (NHDF). Clonetics™ Human Dermal Fibroblasts are available from either adult [[skin]] (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult [[skin]]. They are cryopreserved at the end of primary culture. Applications:* ECM protein analysis* Wound healing* Collagen metabolism* Cosmetics* Skin therapy/models All cells test negative for mycoplasma, bacteria, yeast, and fungi. [[HIV]]-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.<ref>{{REFweb

|url=httpshttp://toolswww.lifetechnologieslonza.com/contentproducts-services/sfsbio-research/manualsprimary-cells/HDFn_manhuman-cells-and-media/fibroblasts-and-media/human-dermal-fibroblasts.pdfaspx |title=Life Technologies HDFn manualHuman dermal fibroblasts

Life Technologies, now a brand of Thermo Fisher Scientific, traces its roots back to 1962 with the creation of Grand Island Biological Company Clonetics™ Dermal Fibroblast Cell Systems contain Normal Human Dermal Fibroblasts (GIBCONHDF) in Grand Island, New York, making serum harvested from horses raised on and optimized media for their land. The original Life Technologies, Incgrowth., a subsidiary of Dexter Corp., was founded in 1983 by Each System can quickly generate NHDF cultures for the merger study of Bethesda Research Laboratories, Inc. and GIBCO Corporation. In 2000, Life Technologies was bought out by Invitrogen. Eight years later, following Invitrogen Corporation’s acquisition disorders of Applied Biosystems Inc.human fibroblasts such as fibrosis, Invitrogen renamed itselfscleroderma, creating the new Life Technologies Corp. In 2014fibrosarcoma, Life Technologies became the fourth premier brand within Thermo Fisher, the world leader in serving sciencexeroderma pigmentosum and histiocytoma.<ref>{{REFweb

|url=httpshttp://wwwbio.lifetechnologieslonza.com/usuploads/entx_mwaxmarketingmaterial/home/aboutLonza_ManualsProductInstructions_TechSheet_-us/news-gallery/company-fact-sheet/company-history_Dermal_Fibroblast_Cell_Systems_NHDF.htmlpdf |title=Life Technologies, company historyLonza Dermal fibroblasts cell systems - techsheet

According to PromoCell's website, the tissue used by PromoCell for the isolation of human cell cultures is derived from donors who have signed an [[informed consent ]] form (this being done by the donor himself, an authorized agent, or a legal agent) which outlines in detail the purpose of the donation and the procedure for processing the tissue. We do not accept or use any tissue without prior signing of the consent documents.