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The preputial sac is lined with [[mucosa]] as is appropriate for an internal organ where moisture is present.<ref name="cold-taylor1999">{{ColdCJ TaylorJR 1999}}</ref> [[Urine]] and other sources of moisture are not harmful to it.
The [[glans penis]] (head of the [[penis]]) is covered and protected from friction, abrasion, [[trauma]], and [[infection]] by the [[foreskin]] while it is in the preputial sac,<ref name="fleiss1998">{{FleissP HodgesF VanHoweRS 1998}}</ref> so it does not accumulate [[keratin]], and maintains its [[Foreskin sensitivity| natural sensitivity]]. It is normal for the [[glans penis]] to be purple in color when the glans has been protected by the [[foreskin]].
Parkash et al. (1973) reported that epithelial debris [exfoliated skin or mucosa] accumulates in the preputial sac and forms [[smegma]].<ref name="parkash1873">{{REFjournal