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The dartos muscle works in conjunction with the cremaster muscle to elevate the testis but should not be confused with the cremasteric reflex.
The dartos fascia keeps the foreskin close to the glans penis throughout life. <ref>{{REFjournal | last=Lakshmanan | first=S | coauthors=Prakash S | title=Human prepuce: some aspects of structure and function | journal=Indian J Surg | date=1980 | volume=44 | issue= | pages=134-7 | url=http://www.cirp.org/library/anatomy/lakshmanan/ | quote= | pubmedID= | pubmedCID= | DOI= | accessdate=2019-09-29}}</ref> In infancy, the dartos fascia operates as a one-way check valve at the tip of the foreskin, allowing urine to pass out, but prohibiting the entry of foreign matter and pathogens.<ref>{{REFjournal
| last=Fleiss
| first=PM